Kraken Collagen Hydrogel 18 mg/mL - Research Use Only
1. Why does clogging occur during extrusion?
Clogging typically results from premature gelation due to inadequate temperature control, extended time in the needle, or shear heating. During handling, ensure that all equipment and components are chilled prior to use. Ensure that the print head is operating within the recommended temperature range.
2. Why is extrusion flow inconsistent or interrupted?
Inconsistent flow is commonly caused by air bubbles introduced during mixing or loading. Slow, controlled mixing and careful priming are recommended. To remove air bubbles, centrifuge the hydrogel with the cap on and syringe facing up. Do not vortex as this could damage or destroy cells and collagen.
3. Why is gelation not occurring?
Gelation occurs best at physiological pH. Check the pH of the reagents used in the preparation. These should be between pH 7 - 7.5.
4. Why do printed constructs collapse or lose shape?
This may result from insufficient support during gelation, rapid extrusion, or low collagen concentrations. A support bath is recommended for Kraken Collagen Hydrogel when making complex constructs. The bath will provide support during printing and curing. Once cured, the construct can be removed from the bath. Weak gels can also arise due to inadequate collagen concentration.
5. Why are cells not growing in the construct?
Common causes of poor cell growth are shear stress during printing and stiffness of the final construct. During printing ensure that the print parameters are set to reduce shear stress. Kraken Collagen Hydrogel extrudes well through a 21-gauge needle. Different cell types prefer different stiffness. Altering the collagen concentration can be used to provide optimal conditions for certain cells.
